| 张怡评,杨婷,阎光宇,等.高效液相色谱法测定海胆黄中岩藻黄质与岩藻黄醇[J].中国海洋药物,2020,39(1):38-42. |
| 高效液相色谱法测定海胆黄中岩藻黄质与岩藻黄醇 |
| Determination of Fucoxanthin and Fucoxanthinol in Sea Urchin gonad by HPLC |
| 投稿时间：2019-03-20 修订日期：2019-08-07 |
| DOI： |
| 中文关键词: 海胆黄 高效液相色谱 岩藻黄质 岩藻黄醇 |
| English Keywords:Sea Urchin gonad HPLC Fucoxanthin Fucoxanthinol |
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| 中文摘要: |
| 目的 建立高效液相色谱法同时测定海胆黄中岩藻黄质及岩藻黄醇的方法。方法 采用高效液相色谱法,色谱柱为Hypersil ODS C18(4.6 mm×250 mm,５μｍ),流动相为甲醇-水(90:10) ,检测波长为449 nm,流速１mL.min－１,进样量10 μＬ。结果 岩藻黄质与岩藻黄醇在此色谱条件下获得良好分离, 岩藻黄质在0.88～56 μg/mL范围内呈良好线性 (y = 102.75x + 4.4072,r = 0.9999, 平均加样回收率为97.76%, RSD 1.88%),岩藻黄醇在0.88～56 μg/mL范围内呈良好线性 (y = 92.906x + 2.2318,r = 0.9999,平均加样回收率为95.28%, RSD 1.85%)。结论 本方法准确、可靠, 可用于海胆等生物样品中岩藻黄质及岩藻黄醇的含量测定。 |
| English Summary: |
| Objective To establish a method for the determination of Fucoxanthin and Fucoxanthinol in Sea Urchin gonad by high performance liquid chromatography ( HPLC)．Methods The determination was carried out on C18 column(4.6 mm×250 mm,５μｍ)． The mobile Phase consisted of Methanol water ( 90: 10) with flow rate of 1.0 mL/min and detection wavelength set at 449nm．Results Fucoxanthin and Fucoxanthinol can be separated well under this chromatographic condition. The linear ranges were 0.88～56 μg/mL for both Fucoxanthin and Fucoxanthinol(Fucoxanthin y = 102.75x + 4.4072,r = 0.9999, Fucoxanthinol y = 92.906x + 2.2318,r = 0.9999)．The average recoveries were 97.76% for Fucoxanthin ( RSD=1.88%) and 95.28% for Fuxanthinol ( RSD=1.85%)． Conclusion The method is accurate and reliable,it can be used for the detection of Fucoxanthin and Fucoxanthol in Sea Urchin gonad and some other biological samples. |
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