| 陈夏雨,仲伟茂,曾奇,等.基于OSMAC策略对深海来源真菌Acrostalagmus luteoalbus SCSIO F457化学多样性的初步研究[J].中国海洋药物,2020,39(4):7-15. |
| 基于OSMAC策略对深海来源真菌Acrostalagmus luteoalbus SCSIO F457化学多样性的初步研究 |
| A Preliminary Study on the Chemical Diversity of the Deep-sea derived fungus Acrostalagmus luteoalbus SCSIO F457 based on OSMAC strategy |
| 投稿时间：2020-02-12 修订日期：2020-03-24 |
| DOI： |
| 中文关键词: 深海真菌 OMSAC 培养调控 次级代谢产物 化学多样性 |
| English Keywords:deep-sea derived fungi OSMAC culture regulation secondary metabolites chemical diversity |
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| 中文摘要: |
| 摘要：目的 采用单菌多次级代谢产物（OSMAC）策略对1株采自南海深海2 801 m沉积环境的白黄笋顶孢霉属真菌Acrostalagmus luteoalbus SCSIO F457进行化学多样性的初步研究。方法 通过在不同培养基、不同pH与不同盐度条件下对菌株进行培养调控并筛选2种适宜发酵条件进行小规模发酵。采用硅胶柱层析、葡聚糖凝胶层析、半制备高效液相等色谱学方法对发酵产物进行化学分离，利用NMR、MS等波谱学技术并结合文献鉴定化合物结构，并对化合物进行初步抗氧化和抗菌活性测试。结果 从菌株SCSIO F457的发酵产物中共新增分离鉴定11个单体化合物，包括paulownin（1）、cyclo(L-Phe-L-Pro)（2）、cyclo(L-Tyr-L-Pro)（3）、cyclo(L-Val-L-Pro)（4）、cyclo(D-Ile-L-Pro)（5）、cyclo(D-Leu-L-Pro)（6）、1-methyoxy-4-(2-hydroxy)ethylbenzene（7）、2-(4-hydroxyphenyl)-ethanol（8）、1-phenylbutane-2,3-diol（9）、3-methoxy-2-methyl-4H-pyran-4-one（10）及3-(hydroxy-acetyl)-1H-indole（11），化合物7表现出较弱的1,1-二苯基-2-苦基肼（1,1-Diphenyl-2-picryl-hydrazyl, DPPH）自由基清除活性。 |
| English Summary: |
| Abstract: Objective Using one strain many compounds (OSMAC) strategy to study the chemical diversity of Acrostalagmus luteoalbus SCSIO F457 collected from a sedimentary environment at a water depth of 2801 m in the South China Sea. Methods The optimal fermentation conditions of the strain were performed by adjusting the culture conditions under different medium, pH, salinity. The compounds were purified by silica gel column, Sephadex LH-20 and semi-preparative HPLC. The structures of compounds were identified by spectroscopic analysis (NMR and MS) as well as comparison with published researches. The antioxidative and antibacterial activities of the products were evaluated by using the Kirby-Bauer test and the 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging method. Results Eleven compounds obtained from the ethyl acetate extracts of strain SCSIO F457 were reported in this study. Their structures were identified as paulownin (1), cyclo(L-Phe-L-Pro) (2), cyclo(L-Tyr-L-Pro) (3)，cyclo(L-Val-L-Pro) (4), cyclo(D-Ile-L-Pro) (5), cyclo(D-Leu-L-Pro) (6), 1-methyoxy-4-(2-hydroxy)ethylbenzene (7), 2-(4-hydroxyphenyl)-ethanol (8), 1-phenylbutane-2,3-diol (9), 3-methoxy-2-methyl-4H-pyran-4-one (10), 3-(hydroxy-acetyl)-1H-indole (11). Compound 7 showed weak DPPH free radical scavenging activity. |
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