| 孙彩霞,唐旭利,李凯,等.1株海洋来源真菌Aspergillus alabamensis次级代谢产物研究[J].中国海洋药物,2022,41(1):63-69. |
| 1株海洋来源真菌Aspergillus alabamensis次级代谢产物研究 |
| Study on the secondary metabolites of a marine fungus Aspergillus alabamensis |
| 投稿时间：2021-02-08 修订日期：2021-05-08 |
| DOI： |
| 中文关键词: 共附生微生物 Aspergillus alabamensis 次级代谢产物 |
| English Keywords:symbiotic microorganisms Aspergillus alabamensis senocndary metabolites |
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| 中文摘要: |
| 目的 对来源于西沙群岛的海绵（Agelas sp.）共附生微生物Aspergillus alabamensis进行菌种鉴定及次级代谢产物的研究。方法 通过提取基因组DNA、PCR扩增技术、测序、ITS序列比对鉴定菌株种属。综合运用硅胶柱层析，薄层色谱，液相色谱分析和半制备HPLC等色谱学方法对该种进行分离纯化，并通过NMR、LC-MS等光谱手段进行相关理化数据的测定，并根据文献对比确定结构。结果 从中分离得到7个单体化合物，分别是ardeemin型生物碱3个：5-N-acetyl-15bβ-hydroxyardeemin(1)、5-N-acetylardeemin(2)、5-N-acetyl-15b-didehydroardeemin(3)；二酮哌嗪生物碱2个：3-benzylidene-3,4-dihydro-4-methyl-lH-l,4-benzodiazepine-2,5-dione(4)、benzodiazepinedione(5)；吲哚类生物碱2个: luteoride E(6)、14-hydroxyterezine D(7)。结论 从该菌株共分离得到7个生物碱类化合物，其中化合物1在30 μmol·L?1浓度下，对H69AR的活性抑制率为15.15 %。 |
| English Summary: |
| Objective To identify the species and study the secondary metabolites of Aspergillus alabamensis that was a symbiotic microorganism of the sponge (Agelas sp.) from the Paracel Islands. Methods The genus of the strain were identified by extracting genomic DNA、PCR、sequencing and comparing with ITS sequence. The silica gel column chromatography, thin layer chromatography, liquid chromatography analysis and scmi-preparative HPLC and other chromatographic methods that they were separated and purified the species and used NMR, LC-MS and other spectral means determined the relevant physical and chemical datas, and defined the structure with literature. Results Seven alkaloids were isolated from the secondary metabolites of A. alabamensis, including three ardeemin alkaloids：5-N-acetyl-15bβ-hydroxyardeemin (1), 5-N-acetylardeemin (2), 5-N-acetyl-15b-didehydroardeemin (3), two diketopiperazine alkaloids: 3-benzylidene-3,4-dihydro-4-methyl-1H-1,4-benzodiazepine-2,5-dione (4), benzodiazepinedione (5); two indole alkaloids: luteoride E (6), 14-hydroxyterezine D (7). Conclusion: A total of seven alkaloids were isolated from this strain, compound 1 exhibited activity against H69AR with the proliferation inhibition rate of 15.15% at concentration level of 30 μmol·L?1. |
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