| 孙继芹,牛庆凤,秦吉,等.盾果藻Carpopeltis affinis多糖的提取分离、理化性质及抗病毒活性研究[J].中国海洋药物,2023,42(2):10-16. |
| 盾果藻Carpopeltis affinis多糖的提取分离、理化性质及抗病毒活性研究 |
| Extraction, Separation, Physicochemical Property and Antiviral Activity of polysaccharides from Carpopeltis affinis |
| 投稿时间：2022-03-15 修订日期：2022-04-05 |
| DOI： |
| 中文关键词: 盾果藻 多糖 结构表征 抗病毒活性 |
| English Keywords:Carpopeltis affinis polysaccharide structure antiviral activity |
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| 中文摘要: |
| 目的 采用不同方法提取盾果藻(Carpopeltis affinis)中多糖,对其理化性质和结构进行分析,并初步评价其抗病毒活性。方法 依次通过冷水、热水(80 ℃)和2% NaOH溶液提取盾果藻多糖,通过硫酸苯酚、离子色谱法、PMP柱前衍生高效液相色谱法等对其总糖、硫酸根含量、单糖组成等进行分析,利用核磁共振波谱技术初步探究其结构。通过CPE和假病毒抑制实验初步探究其抗病毒活性。结果 3种多糖CCAP(冷水提取)、HCAP(热水提取)、ACAP(2% NaOH提取)均主要含硫酸半乳糖,热水浸提的HCAP得率及纯度较高,且主要为τ-卡拉胶。抗病毒活性研究表明HCAP对流感病毒、疱疹病毒和新冠假病毒具有显著的抑制作用,其中对H1N1株Influenza(A/Virginia/ATCC1/2009)的抑制作用最为显著(IC50=0.03 μg.mL-1,SI>66666.6)。结论 热水提取获得的盾果藻多糖为τ-卡拉胶,其对多种病毒均具有显著的抑制作用,本研究结果丰富了海洋糖库,为海洋抗病毒药物的开发提供了理论基础。 |
| English Summary: |
| Objective To extract polysaccharides from Carpopeltis affinis by different methods, investigate their physicochemical properties, structural characteristics and antiviral activities. Methods Three polysaccharides were prepared from a red alga Carpopeltis affinis by sequential extraction with cold water, hot water at 80 ℃ and 2% sodium hydroxide water solution. The total sugar, sulfate content and monosaccharide composition of three polysaccharides were determined by phenol-sulfuric acid, ion chromatography and pre-column derivatization high performance liquid chromatography, respectively. The structure of polysaccharides was characterized by nuclear magnetic resonance spectroscopy. Cytopathic effect (CPE) and pseudovirus inhibition assay were used to preliminary investigate their antiviral activities. Results Three polysaccharides CCAP (cold water), HCAP (hot water at 80 ℃), ACAP (2% sodium hydroxide water solution at 60 ℃) were all mainly composed of sulfated galactans. The yield and purity of HCAP was higher and the NMR data indicated it was τ- carrageenan. The antiviral activity studies have proved that HCAP could inhibit influenza A virus, herpes virus and sever acute respiratory syndrome coronavirus 2 pseudovirus. Among them, the inhibition effect on H1N1 strain Influenza (A/Virginia/ATCC1/2009) was the most significant (IC50=0.03 μg.mL-1,SI>66666.6). Conclusion The hot water extracted fraction was mainly τ-carrageenan and it had a significant inhibitory effect on several kinds of virus. The results enrich the marine carbohydrate library. Meanwhile, it provides the basis for the development of marine antiviral drugs. |
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