| 李中辉,郝宝聪,郑瑶瑶,等.1 株海洋真菌Talaromyces sp. HK1-18次级代谢产物及其抗菌活性研究[J].中国海洋药物,2023,42(6):67-72. |
| 1 株海洋真菌Talaromyces sp. HK1-18次级代谢产物及其抗菌活性研究 |
| Secondary metabolites and antibacterial activity of the marine-derived fungus, Talaromyces sp. HK1-18 |
| 投稿时间：2022-11-01 修订日期：2023-02-11 |
| DOI：10.13400/j.cnki.cjmd.2023.06.020 |
| 中文关键词: 红树林 海洋真菌 Talaromyces sp. 次级代谢产物 抗菌活性 |
| English Keywords:mangrove marine-derived fungus Talaromyces sp. secondary metabolite antibacterial activity |
| Fund Project: |
|
| 摘要点击次数: 1601 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的 研究 1 株分离于海南东寨港红树林自然保护区海桑根际土壤的真菌Talaromyces sp. HK1-18的次级代谢产物及其抗菌活性。方法 利用正、反相硅胶柱层析、凝胶柱层析和半制备高效液相色谱等色谱分离方法,对该菌的次级代谢产物进行分离纯化；利用核磁共振波谱以及参考文献等方法,鉴定化合物的结构；采用MIC法对化合物进行抗菌活性评价。结果 从该真菌中分离获得 8个化合物 (1～8),结构分别鉴定为 penicillide(1)、purpactin A(2)、vermistatin(3)、deoxyfunicone(4)、dankasterone A(5)、fortisterol(6)、过氧化麦角甾醇(7)、radiclonic acid(8)。结论 化合物6为首次从Talaromyces属真菌中分离得到。化合物1和8对多药耐药金黄色葡萄球菌(S. aureus ATCC 43300)显示出较强的抑制活性,MIC值均为0.78 μg/mL。化合物6对耐甲氧西林金黄色葡萄球菌(S. aureus ATCC 33591)显示出较强的抑制活性,MIC值为0.78 μg/mL。 |
| English Summary: |
| Objective To study the secondary metabolites and antibacterial activity of the marine-derived fungus, Talaromyces sp.HK1-18, isolated from the mangrove rhizosphere soil collected from Dongzhaigang Mangrove Nature Reserve, Hainan Province. Methods The secondary metabolites were isolated by silica gel column chromatography (CC), reversed phase silica gel CC, Sephadex LH-20 gel CC and semi-preparative high performance liquid chromatography. The structures were identified by nuclear magnetic resonance spectroscopy (NMR) and compared with the reported spectral data. The antibacterial activity of the compounds was evaluated by MIC method. Results Eight compounds (1～8) were isolated from the fungal strain of HK1-18. The structures were identified as penicillide (1), purpactin A (2), vermistatin (3), deoxyfunicone (4), dankasterone A (5), fortisterol (6), ergosterol peroxide (7), and radiclonic acid (8). Conclusion Compound 6 was isolated from the genus of Talaromyces for the first time. Compounds 1 and 8 showed strong antibacterial activity against multidrug resistant Staphylococcus aureus ATCC 43300 with the same MIC value of 0.78 μg/mL. Compound 6 displayed strong inhibitory activity against methicillin-resistant S. aureus ATCC 33591 with the MIC value of 0.78 μg/mL. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |