| 刘澳莹,张鹏,杨文哲,等.乌贼墨纳米颗粒制备工艺优化及肿瘤光热治疗应用研究[J].中国海洋药物,2024,43(3):19-28. |
| 乌贼墨纳米颗粒制备工艺优化及肿瘤光热治疗应用研究 |
| Study on preparation optimization and application of tumor photothermal therapy of squid ink nanoparticles |
| 投稿时间：2023-02-22 修订日期：2023-04-13 |
| DOI：10.13400/j.cnki.cjmd.2024.03.004 |
| 中文关键词: 乌贼 乌贼墨纳米颗粒 光热转化效率 肿瘤光热治疗 |
| English Keywords:squid squid ink nanoparticles photothermal conversion efficiency photothermal treatment of tumor |
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| 中文摘要: |
| 目的 分离纯化乌贼墨纳米颗粒(squid ink nanoparticles,SINPs),开展材料学表征,考察其肿瘤光热治疗(photothermal therapy,PTT)作用。方法 通过搅拌、超声和离心纯化出SINPs,在近红外激光(Near Infra-Red,NIR)照射下考察其体外光热转化效果,采用CCK-8法测定细胞存活率,通过对小鼠瘤内注射给药的同时给予激光照射研究其体内抑瘤效果。结果 制备的SINPs粒径为(212 ± 1.4) nm,Zeta电位为(-14.5 ± 0.7) mV。测定其光热转化效率为35.5%,说明其可作为一种光热转化剂。800 μg/mL的SINPs在808 nm激光照射下小鼠乳腺癌细胞(4T1)的存活率为67.3%,表明SINPs联合PTT对4T1细胞有一定的细胞毒性,此外,在荷瘤小鼠体内,空白组瘤重为SINPs高+laser组瘤重的2.3倍,说明SINPs联合PTT有一定的抗瘤作用。结论 SINPs联合PTT能显著抑制荷瘤小鼠体内肿瘤组织的生长。作为一种天然纳米材料,SINPs具有良好的肿瘤PTT作用,提示其除传统药理作用外,作为天然、安全的纳米材料或载体,在肿瘤光学治疗领域具有一定应用前景。 |
| English Summary: |
| Objective Squid ink nanoparticles (SINPs) were isolated and enriched to characterize the nanoparticles and investigate the role of photothermal therapy (PTT). Methods SINPs were purified by stirring, ultrasound and centrifugation, and the photothermal transformation effect in vitro was investigated under the irradiation of Near Infra-Red (NIR). The cell viability was determined by CCK-8 method, and the tumor inhibitory effect in vivo was studied by laser irradiation combined with intratumoral injection in mice. Results The particle size of SINPs was (212 ± 1.4) nm and Zeta potential was (-14.5 ± 0.7) mV. The photothermal conversion efficiency was 35.5%, indicating that it can be used as a photothermal agents. The cell viability of mouse breast cancer cells (4T1) was 67.3% under 808 nm laser irradiation with 800 μg/mL SINPs, indicating that SINPs combined with PTT had certain cytotoxicity to 4T1 cells. In addition, the tumor weight of the control group was 2.3 times that of the SINPs +laser group. It indicated that SINPs combined with PTT had certain antitumor effect. Conclusion SINPs combined with PTT can significantly inhibit the growth of tumor tissue in tumor bearing mice. As a natural nanomaterial, SINPs has a good PTT effect on tumor, suggesting that in addition to traditional pharmacological effects, SINPs as a natural and safe nanomaterial has a certain application prospect in the field of tumor optical therapy. |
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