| 杨宁,陈静,吴玉娟,等.海洋小分子XF-1抗肺纤维化的实验研究[J].中国海洋药物,2023,42(5):27-33. |
| 海洋小分子XF-1抗肺纤维化的实验研究 |
| Anti-fibrotic effect and mechanisms of the marine derived XF-1 on Pulmonary Fibrosis in rats |
| 投稿时间：2023-08-13 修订日期：2023-09-16 |
| DOI：10.13400/j.cnki.cjmd.2023.05.012 |
| 中文关键词: 海洋来源小分子 肺纤维化 肺腔灌洗液 炎症因子 胶原沉积 |
| English Keywords:Marine-derived small molecule Pulmonary fibrosis Pulmonary lavage fluid Inflammatory factors |
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| 中文摘要: |
| 目的 探讨海洋来源小分子XF-1对博莱霉素（BLM）诱导肺纤维化模型大鼠的治疗作用。方法 首先通过建立TGF-β诱导肺上皮细胞模型，观察不同浓度XF-1的抑制效果。然后采用气道滴注BLM溶液构建肺纤维化大鼠模型，通过血象分析仪检测大鼠肺泡灌洗液（BALF）5种炎症细胞的数量,利用ELISA检测肺泡灌洗液上清细胞因子和肺组织羟脯氨酸、胶原蛋白含量,通过H&E染色和免疫组化IHC对XF-1改善大鼠肺纤维化效果进行系统评价。结果 结果显示XF-1能够有效抑制TGF-β诱导肺上皮细胞的增殖，而且具有毒性低的优点。XF-1还可显著改善肺纤维化大鼠体重降低的状况，有效降低大鼠肺泡灌洗液上清中的TGF-β1、TNF-α和IL-6等炎症细胞因子的含量，抑制肺组织羟脯氨酸、胶原蛋白的表达水平，显著抑制α-SMA的组织表达，这表明XF-1能够明显改善大鼠肺组织炎症浸润、纤维化以及胶原沉积，可作为抗肺纤维化的候选化合物用于进一步研究。 |
| English Summary: |
| Abstract: Objective To investigate the therapeutic effect of XF-1 on bleomycin-induced pulmonary fibrosis in rats. Methods Firstly, the animal model of pulmonary fibrosis was established by instilling BLM solution into the airway, then the 5 kinds of lymphocytes number in bronchoalveolar lavage fluid (BALF) of rats was counted by hemogram analyzer. Secondly, inflammatory cytokines in the supernatant of BALF and the hydroxyproline as well as the collagen proteins in lung tissue were measured by ELISA. Then the effect of XF-1 on pulmonary fibrosis was evaluated by H&E and Masson staining methods. Results The results showed that XF-1 could significantly improve the lung dysfunction of rats, effectively reduce the contents of inflammatory cytokines such as TGF-β1, TNF-α and IL-6 in the supernatant of rat alveolar lavage fluid, and dramatically inhibit the levels of hydroxyproline and collagen in lung tissue. The inflammatory infiltration, fibrosis and collagen deposition in rat lung tissue were obviously improved. |
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