| 袁华芳,彭志兰,王瑞敏,等.方格星虫多肽对2型糖尿病小鼠血糖和血脂异常的改善作用研究[J].中国海洋药物,2025,44(2):10-16. |
| 方格星虫多肽对2型糖尿病小鼠血糖和血脂异常的改善作用研究 |
| The potential protective Effects of Peptides from Sipunculus nudus Hydrolysate on unconventional plasma glucose level and Dyslipidemia in type 2 diabetes mice |
| 投稿时间：2023-09-08 修订日期：2023-11-02 |
| DOI：10.13400/j.cnki.cjmd.2025.02.009 |
| 中文关键词: 方格星虫 多肽 2型糖尿病 糖脂代谢 氧化应激 |
| English Keywords:Sipunculus nudus polypeptide type 2 diabetes glucose and lipid metabolism oxidative stress |
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| 中文摘要: |
| 目的 探究方格星虫多肽(sipunculus nudus polypeptide,SNP)对链脲佐菌素(streptozotocin,STZ)诱导的糖尿病小鼠的降血糖和降血脂活性,以及胰岛细胞的保护作用。方法 采用腹腔注射链脲佐菌素结合高糖高脂饲料喂养建立2型糖尿病(type 2 diabetes mellitus,T2DM)小鼠模型,不同剂量的SNP连续灌胃给药6周,每周定期监测小鼠体重、饮食量,饮水量、空腹血糖生命体征变化,实验结束后测定小鼠血清中血脂以及胰岛素水平。检测血清中超氧化物歧化酶(superoxide dismutase,SOD)活性与丙二醛(malondialdehyde,MDA)含量,并通过HE染色观察小鼠胰腺组织形态变化。结果6周的SNP给药能降低T2DM小鼠的血糖和调节胰岛素抵抗水平,抑制T2DM小鼠血清中甘油三脂(triacylglycerol,TG)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)的水平,提高糖尿病小鼠高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)的水平,同时对T2DM小鼠的胰腺组织病理改变具有保护作用。此外,SNP给药能提高小鼠血清中超氧化物歧化酶(SOD)的活性,抑制脂质过氧化标记物丙二醛(MDA)的表达。结论 SNP对2型糖尿病小鼠的血糖和血脂变化具有调节作用,可能与其调节糖脂代谢紊乱、改善胰岛素抵抗、氧化应激有关。 |
| English Summary: |
| Objective To investigate effects of Sipunculus nudus nudus polypeptides (SNP) on glucose control and lipid abnormalities and in a type 2 diabetic mice model. Methods Type 2 diabetes (T2DM) mice model was firstly established by successive intraperitoneal injection of Streptozotocin combined with high glucose and high-fat diet. Subsequently, T2DM mice were were administered different doses of SNP for 6 weeks. The body weight, diet and water consumption, as well as fasting blood sugar levels were monitored every week. After the experiment, serum lipids and insulin levels were measured by kits. The activity of superoxide dismutase (SOD) and the content of Malondialdehyde (MDA) in serum were further detected.Results 6 weeks of SNP administration exhibited regulatory effects on fasting blood sugar level increase and regulate insulin resistance. In addition, SNP treatment lowered triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) concentration, but increased the level of High-density lipoprotein cholesterol (HDL-C) in T2DM mice, It also has a protective effect on the pathological changes of pancreatic tissue in T2DM mice. Moreover, SNP administration could improve the activity of SOD) , whereas inhibit the expression of MDA in serum.Conclusion SNP can regulate the changes of blood glucose and lipids in type 2 diabetes mice, which may be related to the regulation of glucose and lipid metabolism disorder, improvement of insulin resistance and oxidative stress. |
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