叶禹秀,梁嘉琦,杨斌,等.北部湾海绵共附生真菌Cladosporium sp. SCSIO 41415次级代谢产物研究[J].中国海洋药物,2025,(5):-.
北部湾海绵共附生真菌Cladosporium sp. SCSIO 41415次级代谢产物研究
Study on the Secondary Metabolites from the Beibu Gulf Sponge-Associated Fungus Cladosporium sp. SCSIO 41415
投稿时间：2024-06-26  修订日期：2024-08-08
DOI：
中文关键词:  海绵共附生真菌  Cladosporium sp  次级代谢产物  抗菌活性
English Keywords:Sponge-associated fungus  Cladosporium sp.  Secondary metabolites  Antibacterial activity.
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作者单位邮编
叶禹秀 中国科学院南海海洋研究所 510301
梁嘉琦 中国科学院南海海洋研究所 
杨斌 中国科学院南海海洋研究所 
刘永宏 中国科学院南海海洋研究所 
周雪峰* 中国科学院南海海洋研究所 510301
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中文摘要:
      目的 研究涠洲岛海绵共附生真菌Cladosporium sp. SCSIO 41415次级代谢产物。方法 采用中压正相柱层析色谱法和半制备高效液相色谱法，对真菌的大米培养基发酵物进行分离和纯化，采用核磁共振、质谱、圆二色谱等现代波、光谱学方法，结合其理化性质和文献数据对比，进行化合物的结构鉴定。结果 从中获得6个体化合物，分别为：butyrolactone VII（1）、butyrolactone I（2）、butyrolactone V（3）、甲酯型莫纳克林K（4）、monacolin K（5）、dehydromonacolin K（6）。对14种病原菌的抗菌筛选发现，仅化合物1和2对藤黄微球菌具有抑制作用，MIC值分别为16 μg·mL–1和8 μg·mL–1。结论 分离鉴定的6个单体化合物均为首次从该菌株发现，其中化合物1和2对藤黄微球菌具有抑制活性。
English Summary:
      Objective To study the secondary metabolites of the sponge-associated fungus Cladosporium sp. SCSIO 41415 collected from the Weizhou Island. Methods Medium-pressure normal-phase column chromatography and semi-preparative high-performance liquid chromatography were used to separate, and purify the metabolites from the fungal rice fermentation extract. Modern spectroscopic methods such as nuclear magnetic resonance, mass spectrometry and electronic circular dichroism, along with their physicochemical properties, were used to identify the compounds. Results Six compounds were obtained and were identified as butyrolactone VII (1), butyrolactone I (2), butyrolactone V (3), methyl monacolin K (4), monacolin K (5), and dehydromonacolin K (6). The antibacterial screening against 14 pathogens showed that only compounds 1 and 2 had inhibitory effects on Micrococcus luteus, with MIC values of 16 μg·mL–1and 8 μg·mL–1, respectively. Conclusion The obtained six compounds were discovered from this strain for the first time from. Compounds 1 and 2 showed antibacterial activities against Micrococcus luteus.
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