| 蔡沛玲,马晓青,魏宝红,等.基于HPLC指纹图谱和指标成分定量结合化学计量学的乌贼墨质量评价研究[J].中国海洋药物,2026,(3):-. |
| 基于HPLC指纹图谱和指标成分定量结合化学计量学的乌贼墨质量评价研究 |
| Quality evaluation of Cuttlefish ink based on HPLC fingerprint and component quantification combined with chemometric analysis |
| 投稿时间：2025-02-26 修订日期：2025-03-31 |
| DOI： |
| 中文关键词: 乌贼墨 HPLC指纹图谱 含量测定 化学计量学 PTCA |
| English Keywords:cuttlefish ink HPLC fingerprinting content determination chemometrics PTCA |
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| 中文摘要: |
| 目的 通过HPLC(高效液相色谱法)指纹图谱、指标成分含量测定与化学计量学相结合的方法,为构建乌贼墨质控体系及探索其潜在质量标志物提供参考和依据。方法 采用Waters Atlantis T3(4.6×250 mm,5 μm),以5%乙腈/甲醇-95%磷酸二氢钠缓冲盐为流动相,检测波长275 nm,建立10批不同来源的乌贼墨HPLC指纹图谱及测定2,3,5-三羧酸吡咯(PTCA)含量,同时对易混品种进行检查。结果 10批乌贼墨的指纹图谱相似度为0.938~0.999；共标定5个共有峰,指认其中1个共有成分为PTCA,含量为0.62%~1.31%；而2批易混品种枪乌贼墨的相似度仅为0.552~0.576,PTCA含量仅为0.30%~0.32%。指纹图谱和含量测定结果表明不同来源乌贼墨中化学成分存在明显差异,能较好区分地方中药材标准中的乌贼墨品种及其易混品种。通过CA、PCA和OPLS-DA可将10批乌贼墨和2批易混淆品明显分为2类； OPLS-DA表明共有峰峰3和峰5(PTCA)可能是影响乌贼墨质量的差异标志物。结论 通过建立乌贼墨HPLC指纹图谱、测定PTCA含量,并结合化学计量学评价了不同来源乌贼墨质量,为乌贼墨质量标准的研究及其潜在质量标志物的研究提供依据。 |
| English Summary: |
| Objective To analyze the quality of cuttlefish ink by HPLC fingerprint, component quantification and chemometrics, as well as to provide a reference and basis for the quality control and assessment system construction of cuttlefish ink and the exploration of its potential quality markers. Methods HPLC fingerprints were established for 10 batches of cuttlefish ink sourced from various locations, and pyrrole 2,3,5-tricarboxylic acid (PTCA) was quantified. Additionally, an assessment of easily confusable species was conducted. Using a Waters Atlantis T3 (4.6×250 mm,5 μm), a mobile phase consisting of 5% acetonitrile/ methanol-95% sodium dihydrogen phosphate buffer was employed,and with a detection wavelength of 275 nm. Results The similarity of the fingerprints of 10 batches of cuttlefish ink was of 0.938-0.999; 5 common peaks were calibrated, with one of the components identified as PTCA, which had a concentration of 0.62%-1.31%; In contrast, the similarity of the fingerprints for 2 batches of easily mixed squid ink varieties ranged 0.552-0.576, with PTCA content measured at only 0.30%-0.32%. The results of fingerprinting and content determination revealed significant differences in the chemical compositions of cuttlefish ink from various sources. These difference could effectively distinguish between cuttlefish ink varieties in the context of local herbal medicines standards and the easily mixed varieties.10 batches of cuttlefish ink and 2 batches of easily confusable species were clearly categorized into two distinct groups using CA, PCA and OPLS-DA; OPLS-DA further indicated that the peak 3 and peak 5 (PTCA) may be the differential markers influencing the quality of cuttlefish ink. Conclusion The quality of cuttlefish ink from different sources was evaluated using established HPLC fingerprints, the concentration of PTCA, and combining with chemometrics, so as to provide a foundation for developing quality standards of cuttlefish ink and identifying its potential quality markers. |
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