| 乔博,高钰,姜帅.昆布多糖改性微针的制备与表征[J].中国海洋药物,2026,(4):-. |
| 昆布多糖改性微针的制备与表征 |
| Preparation and characterization of laminarin-modified microneedles |
| 投稿时间：2025-04-18 修订日期：2025-05-20 |
| DOI： |
| 中文关键词: 昆布多糖 透明质酸 微针 生物相容性 |
| English Keywords:Laminarin hyaluronic acid microneedles biocompatibility |
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| 中文摘要: |
| 目的 以透明质酸(HA)、聚乳酸(PLA)和昆布多糖(LAM)为微针制备基质,通过工艺优化获得机械性能良好的微针阵列,并评估其透皮性能和生物相容性。方法 以HA为针尖基质材料,昆布多糖改性PLA为背衬层材料,通过分层浇筑制备复合微针。采用照相机微距模式和扫描电镜观察微针形貌,力学测试评估压缩断裂性能,苏木精-伊红(H E)染色、台盼蓝染色和共聚焦显微镜分析透皮效果,并通过CCK-8法检测细胞相容性。结论 制备的HA可分离微针阵列形态完整,针尖高度约750 μm；压缩断裂强度满足透皮要求,能有效穿透皮肤角质层。与小鼠成纤维细胞共培养24小时后,细胞活力保持良好,证实其具有良好的生物相容性。本研究为基于HA微针的安全高效经皮给药系统提供了新策略。 |
| English Summary: |
| Objective Utilizing hyaluronic acid (HA), polylactic acid (PLA), and laminarin (LAM) as the microneedle matrix materials, a mechanically robust microneedle array was successfully developed through process optimization, with subsequent evaluation of its transdermal performance and biocompatibility. Methods A composite microneedle system was fabricated via a layer-by-layer casting method, employing HA as the tip matrix material and laminarin-modified PLA as the backing layer material. The morphology of the microneedles was examined using macro-mode photography and scanning electron microscopy (SEM). Mechanical compression testing was conducted to assess fracture resistance, while hematoxylin-eosin (H E) staining, trypan blue staining, and confocal microscopy were employed to analyze transdermal penetration efficiency. Additionally, cell compatibility was evaluated using the CCK-8 assay. Results The prepared HA-based separable microneedle array exhibited intact structural integrity, with a needle height of approximately 750 μm. The compressive fracture strength met transdermal requirements, enabling effective penetration of the stratum corneum. After 24 hours of co-culture with mouse fibroblasts, cell viability remained high, confirming excellent biocompatibility. This study provides a novel strategy for developing safe and efficient HA-based transdermal drug delivery systems. |
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