| 朱丽娜,房孝妍,姜帅.负载小檗碱的壳聚糖温敏型水凝胶的制备与表征[J].中国海洋药物,2026,(4):-. |
| 负载小檗碱的壳聚糖温敏型水凝胶的制备与表征 |
| Preparation and characterization of berberine-loaded thermo-sensitive chitosan hydrogel |
| 投稿时间：2025-04-18 修订日期：2025-05-17 |
| DOI： |
| 中文关键词: 药物递送 小檗碱 壳聚糖 水凝胶 温度响应性 |
| English Keywords:drug delivery berberine chitosan hydrogel thermoresponsive |
| Fund Project: |
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| 中文摘要: |
| 目的 构建一种负载小檗碱的壳聚糖温敏型水凝胶，并系统评估其理化性质、生物相容性及作为药物递送系统的潜力。方法 以壳聚糖为基质，β-甘油磷酸钠（β-GP）为交联剂，构建含不同浓度β-GP的壳聚糖水凝胶体系，并筛选最优成胶配比。通过流变学测试、扫描电镜、溶胀降解等实验对水凝胶的温敏性、力学性能与微观结构等进行表征，同时考察其对人脐静脉内皮细胞的细胞相容性。结果 随着β-GP浓度的增加，水凝胶的成胶时间明显缩短，最低可至61 s，且在37 ℃下均具有良好的温敏性和机械稳定性。选用终质量浓度为0.12 g/mL的β-GP体系负载小檗碱，该载药水凝胶保持良好的注射性与温敏凝胶性。扫描电镜结果显示，水凝胶具有多孔网络结构，有利于药物的缓释释放。溶胀率达230%以上，7 d内降解率约70%，表现出优良的组织相容性。细胞实验结果表明，水凝胶对人脐静脉内皮细胞无明显毒性，细胞活力维持在80%以上。结论 所制备的负载小檗碱的壳聚糖温敏型水凝胶具有良好的理化性能和生物相容性，适用于小檗碱的局部递送，展现出作为中药控释载体的应用潜力。 |
| English Summary: |
| Objective To develop a thermo-sensitive chitosan hydrogel loaded with berberine and systematically evaluate its physicochemical properties, biocompatibility, and potential as a drug delivery system. Methods Chitosan was used as the hydrogel matrix and β-glycerophosphate (β-GP) as the crosslinker to construct hydrogel systems with varying concentrations of β-GP. The optimal gelation ratio was screened based on gelation time. The thermo-sensitivity, mechanical properties, and microstructure of the hydrogels were characterized by rheological analysis, scanning electron microscopy (SEM), and swelling/degradation tests. Cytocompatibility was assessed using human umbilical vein endothelial cells (HUVECs). Results Increasing β-GP concentration significantly reduced the gelation time, with the shortest time reaching 61 s. All hydrogels exhibited good thermo-sensitivity and mechanical stability at 37 °C. A hydrogel system with a β-GP final concentration of 0.12 g.mL-1 was selected to load berberine. The resulting hydrogel maintained excellent injectability and thermo-responsive gelation behavior. SEM images showed a porous network structure favorable for sustained drug release. The hydrogel exhibited a swelling ratio of over 230% and a degradation rate of approximately 70% within 7 days, demonstrating good tissue compatibility. Cytotoxicity assays showed no significant toxicity to HUVECs, with cell viability maintained above 80%. Conclusion The prepared berberine-loaded thermo-sensitive chitosan hydrogel exhibits excellent physicochemical properties and biocompatibility, making it suitable for localized delivery of berberine and showing promising potential as a controlled-release carrier for traditional Chinese medicine. |
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