| 孙雯婧,张玉鑫,程亚杰,等.60株深海来源真菌发酵提取物的防污活性与药理活性评价研究[J].中国海洋药物,2027,(1):-. |
| 60株深海来源真菌发酵提取物的防污活性与药理活性评价研究 |
| Evaluation of Antifouling and Pharmacological Activities of 60 Fermentation Extracts from Deep-Sea-Derived Fungal Strains |
| 投稿时间：2025-09-24 修订日期：2025-11-12 |
| DOI： |
| 中文关键词: 深海真菌 马里亚纳海沟 防污活性 药理活性 |
| English Keywords:deep-sea-derived fungi Mariana Trench antifouling activity pharmacological activity |
| Fund Project: |
|
| 摘要点击次数: 60 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的 研究马里亚纳海沟珊瑚来源的深海真菌发酵提取物的防污、抗菌和细胞毒活性，挖掘其在海洋天然防污剂开发和药物研发中的发展潜力。方法 通过抗硅藻附着和抑制贻贝分泌足丝评价模型进行防污活性筛选，分别采用微量稀释法进行抗菌活性筛选、四唑盐比色法进行细胞毒活性筛选。结果 对马里亚纳海沟珊瑚来源的60株深海真菌的发酵提取物进行防污活性和药理活性评价。结果显示，在20 μg/mL时，有10株真菌的发酵提取物对短小舟形藻Navicula exigua抑制率达50%以上，8株真菌的发酵提取物对三角褐指藻Phaeodactylum tricornutum的抑制率达50%以上，其中Aspergillus subramanianii KXHG-03-41和Aspergillus bridgeri KXHG-57-34防污活性显著，其发酵提取物对短小舟形藻N. exigua的EC50值分别为1.08和1.66 μg/mL，对三角褐指藻P. tricornutum的EC50值分别为1.76和2.23 μg/mL。此外，在50 μg/mL时，约1/2的真菌发酵提取物对人结肠癌细胞HT29或人膀胱癌细胞T24的抑制率超过50%。结论 马里亚纳海沟珊瑚来源的深海真菌具有广泛的生物活性，在海洋天然防污剂开发和药物研发中具有巨大的发展潜力。 |
| English Summary: |
| Objective To investigate the antifouling, antimicrobial, and cytotoxic activities of fermentation extracts from deep-sea-derived fungi associated with Mariana Trench corals, and to further explore their potential. Methods Antifouling activity was evaluated using assays against diatom adhesion and mussel byssus secretion. Antimicrobial activity and cytotoxic activity were screened by the microdilution method and the MTT assay, respectively. Results The fermentation extracts of 60 deep-sea fungal strains derived from corals in the Mariana Trench were evaluated for their antifouling and pharmacological activities. The results showed that at a concentration of 20 μg/mL, the fermentation extracts of 10 fungal strains exhibited an inhibition rate of over 50% against the diatom Navicula exigua, and those of 8 fungal strains showed an inhibition rate of more than 50% against Phaeodactylum tricornutum. Among them, Aspergillus subramanianii KXHG-03-41 and Aspergillus bridgeri KXHG-57-34 displayed significant antifouling activity against N. exiguawith with EC50 values of 1.08 μg/mL and 1.66 μg/mL, respectively, and the EC50 values against P. tricornutum of 1.76 μg/mL and 2.23 μg/mL, respectively. In addition, at 50 μg/mL, about half of the fermentation extracts from fungal strains exhibited an inhibition rate of over 50% against either human colon cancer cells (HT29) or human bladder cancer cells (T24). Conclusion The deep-sea fungi derived from Mariana Trench corals possessed extensive bioactivities, with significant potential for application as environment-friendly marine antifouling agents and as valuable resources for pharmaceutical research and drug discovery. |
| 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |