| 王欣怡,蒲凡琪,吴艳平,等.海洋Streptomyces sp. ZSA36中抗生物膜次级代谢产物研究[J].中国海洋药物,2026,(6):-. |
| 海洋Streptomyces sp. ZSA36中抗生物膜次级代谢产物研究 |
| Study on Anti-biofilm Secondary Metabolites from Marine Streptomyces sp. ZSA36 |
| 投稿时间：2025-10-15 修订日期：2025-11-07 |
| DOI： |
| 中文关键词: 海洋沉积物 Streptomyces sp. 次级代谢产物 抗生物膜 |
| English Keywords:Marine sediment Streptomyces sp. Secondary metabolites Anti-biofilm |
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| 中文摘要: |
| 目的 研究海洋沉积物来源链霉菌Streptomyces sp. ZSA36中具有抗生物膜活性的次级代谢产物。方法 通过硅胶柱色谱、Sephadex LH-20凝胶柱色谱以及制备型高效液相色谱等技术对该菌的发酵提取物进行分离纯化；运用核磁共振(NMR)和高分辨电喷雾电离质谱(HRESIMS)等现代波谱学方法,确定化合物平面结构；结合文献数据对比分析以及量子计算化学确定化合物的绝对构型。采用微量板生物膜测定法对化合物进行抗生物膜活性评价；采用磺酰罗丹明B蛋白染色法(SRB法)对化合物进行细胞毒活性测试。结果 从菌株中共分离得到4个化合物,其中包括2个新天然产物,即N-Acetyl-3-hydroxy-4-methoxy-5-methyl-L-phenylalanine(1)、Acetyl-L-phenylalanyl-L-tryptopha(2)以及两个已知化合物N-acetyl-3-(4-hydroxy-3-methoxyphenyl)-L-alanine acetate(3)、N-acetyl-L-tyrosine(4)。微量板生物膜及细胞毒活性测试结果显示化合物1、2和4均有明显抗生物膜活性且无细胞毒活性。 结论 本研究从海洋沉积物来源链霉菌中分离获得3种具有抗生物膜活性且低细胞毒性的次级代谢产物,丰富了海洋微生物来源抗生物膜活性天然产物的研究内容,为开发新型抗生物膜药物提供了潜在的先导化合物和研究基础。 |
| English Summary: |
| Objective To investigate the secondary metabolites with anti-biofilm activity from the marine sediment-derived Streptomyces sp. ZSA36. Methods The fermentation extract was isolated and purified using techniques including silica gel column chromatography, Sephadex LH-20 gel chromatography, and preparative high-performance liquid chromatography. The planar structures of the compounds were elucidated by spectroscopic methods such as nuclear magnetic resonance and high-resolution electrospray ionization mass spectrometry. Their absolute configurations were determined by comparing with literature data and quantum chemical calculations. The anti-biofilm activities of the compounds were evaluated using the microtiter plate biofilm assay, and their cytotoxic activities were tested by the sulforhodamine B assay. Results Four compounds were isolated from the strain, including two new natural products: N-Acetyl-3-hydroxy-4-methoxy-5-methyl-L-phenylalanine (1) and Acetyl-L-phenylalanyl-L-tryptophan (2), along with two known compounds: N-acetyl-3-(4-hydroxy-3-methoxyphenyl)-L-alanine acetate (3) and N-acetyl-L-tyrosine (4). In biofilm and cytotoxicity assays, compounds 1, 2 and 4 showed significant anti-biofilm activity and no detectable cytotoxicity. Conclusion This study led to the isolation of three low-cytotoxicity secondary metabolites with anti-biofilm activity from a marine sediment-derived Streptomyces strain. The findings enrich the research on anti-biofilm secondary metabolites from marine sediment-derived Streptomycetes and provide a foundation for the investigation of natural anti-biofilm products. |
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