| 王远见,沈明曦,张熳,等.海洋来源真菌Aspergillus fumigatus PJ-Y01的次级代谢产物及其活性研究[J].中国海洋药物,2025,44(6):19-26. |
| 海洋来源真菌Aspergillus fumigatus PJ-Y01的次级代谢产物及其活性研究 |
| Study on the secondary metabolites and activities of the marine-derived fungus Aspergillus fumigatus PJ-Y01 |
| 投稿时间：2025-09-23 修订日期：2025-10-20 |
| DOI：10.13400/j.cnki.cjmd.2025.06.008 |
| 中文关键词: 海洋真菌 Aspergillus fumigatus 次生代谢产物 抗菌 抗氧化 |
| English Keywords:Marine fungi Aspergillus fumigatus Secondary metabolites Anti-bacteria Antioxidant |
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| 中文摘要: |
| 目的 对中国盘锦海泥真菌Aspergillus fumigatus PJ-Y01发酵液中次生代谢产物进行分离鉴定并探究其抗氧化和抗菌活性。方法 采用正相硅胶柱色谱、反相ODS色谱、Sephadex LH-20柱色谱、高效液相色谱技术对海洋来源曲霉属真菌烟曲霉Aspergillus fumigatus PJ-Y01的次级代谢产物进行分析，利用紫外光谱、质谱以及核磁共振波谱进行结构鉴定，通过抗氧化以及对金黄色葡萄球菌和大肠杆菌的抑制实验评价其活性。结果 从真菌Aspergillus fumigatus PJ-Y01次生代谢产物中分离纯化得到10个生物碱类化合物，其中有1个新化合物，分别为fumiquinazoline T（1）、fumiquinazoline F（2）、prelapatin B（3）、（-）-alantrypinone（4）、fumipuinazoline J（5）、3′-（4-Oxoquinazolin-3-yl） spiro[1H-indole-3, 5′-oxolane]-2, 2′-dione（6）、isochaetominine（7）、chaetominine（8）、（3S, 8S, 9S, 12S, 18S）-8, 9-dihydroxyspirotryprostatin A（9）、fumitremorgin C（10）。结论 化合物1为新化合物，活性研究发现化合物2、3、5有较强的ABTS+自由基清除能力，IC50分别为61.02、50.57、31.95 μg/mL。化合物4对大肠杆菌有中等抑制作用，化合物9对金黄色葡萄球菌具有较强抑制活性，为新型抗菌以及抗氧化药物的设计提供理论基础。 |
| English Summary: |
| Objective Identifying secondary metabolites from the fermentation liquid of the marine fungus Aspergillus fumigatus PJ-Y01 from Panjin, China, and exploring its antioxidant and antibacterial activities. Methods Using forward silica gel column chromatography, reversed-phase ODS chromatography, Sephadex LH-20 column chromatography, and high-performance liquid chromatography techniques, the secondary metabolites of the marine-derived fungus Aspergillus fumigatus PJ-Y01 were analyzed. Structural identification was conducted using UV spectroscopy, mass spectrometry, and nuclear magnetic resonance spectroscopy. Its activity was evaluated through antioxidant assays and inhibition activity experiments against Staphylococcus aureus and Escherichia coli. Results Ten alkaloid compounds were isolated and purified from the secondary metabolites of the fungus Aspergillus fumigatus PJ-Y01, among which there is one new compound. The structure of these compounds was identified as fumiquinazoline T （1）, fumiquinazoline F （2）, prelapatin B （3）, （-）-alantrypinone （4）, fumiquinazoline J （5）, 3′-（4-Oxoquinazolin-3-yl） spiro[1H-indole-3, 5′-oxolane]-2, 2′-dione （6）, isochaetominine （7）, chaetominine （8）, （3S, 8S, 9S, 12S, 18S）-8, 9-dihydroxyspirotryprostatin A （9）, and fumitremorgin C （10）. Conclusion Compound 1 is new. Activity research has found that compounds 2, 3, and 5 possess strong scavenging abilities against the ABTS+ free radical, with IC50 values of 61.02, 50.57, and 31.95 μg/mL, respectively. While compound 4 exhibits a moderate inhibitory effect on Escherichia coli, and compound 9 displays strong inhibitory activity against Staphylococcus aureus, providing a theoretical basis for the design of new antibacterial and antioxidant drugs. |
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